Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

Table 1 Candidate reference and target genes.

Candidate Reference Genes
Symbol	Name	Function	Assay ID^&	r²	E
ACTB	Actin, beta	Structural protein	Hs99999903_m1	0.997	1.97
B2M	Beta-2-microglobulin	Beta chain of MHC I	Hs99999907_m1	0.999	2.00
CTBP1	C-terminal binding protein 1	Involved in cellular proliferation.	Hs00179922_m1	0.998	2.09
GAPDH	Glyceraldehydes-3-phosphate dehydrogenase	Glycolysis and gluconeogenesis	Hs99999905_m1	0.998	2.00
GOLGA1	Golgi autoantigen, golgin subfamiliy A, 1	Unknown function	Hs00608118_m1	1.000	1.96
PGK1	Phosphoglycerate kinase 1	Glycolysis	Hs99999906_m1	0.997	2.07
PPIA	Cyclophilin A (Peptidylprolyl isomerase A)	Protein folding	Hs99999904_m1	0.996	2.08
PPIB	Cyclophilin B (Peptidylprolyl isomerase B)	Associated with the secretory pathway	Hs00168719_m1	0.996	2.00
SDHA	Succinate dehydrogenase complex, subunit A	Electron transporter	Hs00417200_m1	0.998	2.10
TMBIM4	Transmembrane BAX inhibitor motif containing 4	Involved in apoptosis	Hs00211390_m1	1.000	2.00
TBP	TATA box binding protein	Transcription factor	Hs99999910_m1	0.999	2.10
UBC	Ubiquitin C	Protein degradation	Hs00824723_m1	0.999	2.05
Target Genes
Symbol	Name	Function	Assay ID	r ²	Efficiency*
IL10	Interleukin 10	Antiinflammatory cytokine	Hs00174086_m1	0.989	2.01
TNF-α	Tumor necrosis factor	Proinflammatory cytokine	Hs00174128_m1	0.997	2.08

r², Square of Pearson's correlation coefficient. E, Efficiency as calculated from 10-fold serial dilutions. ^&Assay IDs from Applied Biosystems

ISSN: 1471-2172