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Table 2 Descriptive statistics of candidate reference and target genes as calculated by BestKeeper.

From: Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

Candidate Reference Genes

 

GM [Cq]

AM [Cq]

Min [Cq]

Max [Cq]

SD [± Cq]

CV [% Cq]

ACTB

23.26

23.30

21.05

28.52

0.92

3.95

B2M

21.55

21.55

20.62

22.92

0.40

1.87

CTBP1

30.80

30.84

27.91

36.19

1.25

4.06

GAPDH

23.89

23.91

22.87

26.86

0.58

2.43

GOLGA1

33.11

33.14

30.19

36.67

0.99

3.00

PGK1

26.08

26.09

24.08

27.66

0.57

2.17

PPIA

23.70

23.70

23.02

24.50

0.22

0.91

PPIB

26.45

26.45

25.28

27.95

0.37

1.40

SDHA

25.61

25.63

24.33

29.06

0.80

3.11

TBP

30.35

30.36

28.93

33.11

0.66

2.18

TMBIM4

29.25

29.27

27.23

31.16

0.80

2.74

UBC

26.68

26.68

25.74

28.09

0.47

1.76

Target genes

 

GM [C q ]

AM [C q ]

Min [C q ]

Max [C q ]

SD [± C q ]

CV [%C q ]

IL10

31.16

31.27

27.98

36.68

2.26

7.24

TNF-α

26.68

26.89

22.52

31.80

3.28

12.21

  1. Descriptive statistics were calculated for all 24 samples. Cq, quantification cycle; GM, geometric mean; AM, arithmetic mean; Min, minimum; Max, maximum; SD, standard deviation;CV, coefficient of variation.
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BMC Immunology

ISSN: 1471-2172

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