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Table 5 Expression level changes of TNF-α and IL10 in LPS-stimulated monocytes based on different normalization strategies.

From: Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

TNF-α mRNA

 

ACTB

GAPDH

gN comb

NF comb

 

fold change

p-value

fold change

p-value

fold change

p-value

fold change

p-value

Nm LPS stim/non-stim

172

< 0.05

315

< 0.05

104

< 0.05

158

< 0.05

Ec LPS stim/non-stim

480

< 0.05

596

< 0.05

192

< 0.05

288

< 0.05

IL10 mRNA

 

ACTB

GAPDH

gN comb

NF comb

 

fold change

p-value

fold change

p-value

fold change

p-value

fold change

p-value

Nm LPS stim/non-stim

9

< 0.05

16

< 0.05

6

< 0.05

8

< 0.05

Ec LPS stim/non-stim

13

< 0.05

17

< 0.05

6

< 0.05

7

< 0.05

  1. P-values were assessed by Wilcoxon matched pairs signed ranks test. Paired Student's t test was performed as well and resulted in highly similar findings (data not shown).
  2. gN comb, normalization based on PPIB, B2M and PPIA as suggested by geNorm; NF comb, normalization based on TBP and B2M as suggested by Normfinder; Nm, N. meningitides; Ec, E. coli; stim, stimulated.