Protective effects in ASCIA mice were associated with enhanced production of IL-4 and IL-10 and reduced production of IFN-γ. 56 days after CII immunization in ESCIA mice, ASCIA mice and CIA mice, splenocytes were stimulated by anti-CD3 and anti-CD28 antibodies for 72 hrs. The cytokine contents for IL-4 (A), IFN-g (B), IL-10 (C) and IL-17 (D) were measured by ELISA as described in the Materials and Methods. Shown are samples from individual mouse combined from two or three separately performed experiments. Asterisks* and ** represented P < 0.05 and P < 0.01 respectively by one-way ANOVA with Bonferroni multiple comparison test.