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Figure 2 | BMC Immunology

Figure 2

From: YopH inhibits early pro-inflammatory cytokine responses during plague pneumonia

Figure 2

YopH phosphatase activity is required for Y. pestis virulence. A) Complementation of the yopH mutation leads to similar levels of YopH expression in vitro. Various strains of Y. pestis were grown under type-three secretion system inducing conditions and then the supernatant was subjected SDS-PAGE followed by immuno-blotting with rabbit-polyclonal anti-YopH antibodies. Immunoreactive protein bands were detected by enhanced chemiluminescence. Lanes: 1) molecular weight markers, 2&3) CO92, 4) CO92+pCR2.1, 5) CO92ΔyopH 6) CO92ΔyopH+ pAMC-1 (YopH). Note multiple bands are due to Pla mediated proteolysis. B) Whole cell extracts of cells grown under type-three secretion system inducing conditions. Lanes: 1) molecular weight markers, 2) CO92, 4) CO92+pCR2.1, 5) CO92ΔyopH 6) CO92ΔyopH+ pAMC-1 (YopH). C) Complementation of the yopH mutation restores virulence in vivo. CD-1 mice were infected IN with 2 × 104 CFU of various Y. pestis strains. Mice infected IN with CO92 (υ) or CO92+pCR2.1 (() succumbed rapidly to infection. Mice infected IN with CO92ΔyopH+pAMC-1 (σ), YopH, died with similar kinetics. However, mice infected IN with CO92ΔyopH+pAMC-2 ((), yopH-C403A, survived infection. Data is representative of two independent experiments with 10 mice/group.

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