Figure 1

In vitro expansion of PBMC enriched CD56+ cells. NK cells before and 2 weeks after expansion by co-culture with K562-mb15-41BBL (D2NK) cells were stained with anti-CD56-PE and anti-CD3-FITC antibodies as the markers for NK cells and T cells, and then analyzed by flow cytometry. The UL quadrant counts (NK cells) are 9.60% and 91.20% for PBMC-Day 0, D2NK-Day 14 respectively; The LR quadrant counts (T cells) are 53.22% and 6.60% for PBMC-Day 0, D2NK-Day 14 respectively. PBMC isolated from the Buffy coat were either cultured freshly, or after frozen storage in liquid nitrogen, with equivalent results. The data presented in this figure is typical of 12 expansions made from the PBMC of 5 different donors. Averaged over these 12 experiments, NK cells were 10.05% ± 4.42 and T cells 52.44% ± 6.71 CD3+ before expansion. After expansion, NK cells were 85.25% ± 12.53 and T cells 12.12% ± 5.52 CD3+.