Figure 5

Functional properties of monocyte-derived macrophages from young and old volunteers. Macrophages derived from freshly isolated PBMC from n = 12 volunteers aged <30 years (<30) and from n = 10 volunteers aged >30 years (>30) were cultured in medium containing autologous serum for 24 h and then stimulated with 1 μg/ml LPS for additional 24 h or left untreated. (A) Macrophages display an activated, round-shaped morphology after LPS stimulation, but no overt morphological differences were detected between macrophages from young or old adults. (B) Bar graphs show mean cytokine and chemokine levels (in pg/mL) in the supernatant at day 2, error bars represent SEM.