Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

Table 1 Summary of Hamster cDNA sequences

Hamster Gene	Source of cDNA	Cloned cDNA fragment size	Percent nucleotide identity of hamster cDNA compared to ortholog ¹
			Rattus norvegicus	Sigmodon hispidus	Peromyscus maniculatus	Mus musculus	Homo sapiens
IL-13	Lymph node from hookworm infected hamster	177 bp	81%	85%	83%	80%	76%*
IL-21	Spleen from hookworm infected hamster	262 bp	90%	N/A ²	95%	92%	84%
CCL17	Hamster cDNA library	344 bp	89%	N/A	N/A	86%	77%
CCL22	Hamster cDNA library Lymph node from	310 bp	88%	N/A	N/A	89%	82%
CCR4	hookworm infected hamster Lymph node from	1084 bp	89%	N/A	N/A	90%	83%
FOXP3	hookworm infected hamster	581 bp	93%	N/A	92%	90%	85%

¹ Identity percentage of cross-species identity determined by Basic Local Alignment Search Toll (BLAST) from the National Center for Biotechnology Information (NCBI) using the Nucleotide collection (nr/nt) database and the discontiguous megablast algorithm in all instances except for comparison of hamster to human IL-13, where the Blastn algorithm for somewhat similar sequences was used. The percent identities excluded the primer sequences.
² N/A: sequences not available in NCBI.

ISSN: 1471-2172