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Table 3 GeneBank accession number, primer and probe concentrations for hamster Duplex real-time RT PCR assays

From: Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

Hamster target gene GeneBank number accession Specific forward and reverse primer concentrations
(nM)
Specific probe concentration
(nM)
γ-actin Primers concentration
(nM)
γ-actin Probe
concentration
(nM)
IL-10 AF046210 700 100 80 80
IL-4 AF046213 650 100 65 100
IFN-γ AF034482 600 100 100 100
IL-13 FJ548846 700 100 100 100
IL-21 FJ664142 600 100 100 100
TGF-β1 AF046214 600 100 150 100
CCL17 FJ664143 600 100 100 100
CCL22 FJ664144 650 100 100 100
CCR4 FJ664145,FJ664146,FJ664147 600 100 100 100
FoxP3 FJ664148 650 100 100 100
TNF-α AF046215 600 100 100 100
IL-12p40 AF046211 750 100 100 100
γ -actin FJ664149 - - - -