Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

Table 3 GeneBank accession number, primer and probe concentrations for hamster Duplex real-time RT PCR assays

Hamster target gene	GeneBank number	accession Specific forward and reverse primer concentrations (nM)	Specific probe concentration (nM)	γ-actin Primers concentration (nM)	γ-actin Probe concentration (nM)
IL-10	AF046210	700	100	80	80
IL-4	AF046213	650	100	65	100
IFN-γ	AF034482	600	100	100	100
IL-13	FJ548846	700	100	100	100
IL-21	FJ664142	600	100	100	100
TGF-β1	AF046214	600	100	150	100
CCL17	FJ664143	600	100	100	100
CCL22	FJ664144	650	100	100	100
CCR4	FJ664145,FJ664146,FJ664147	600	100	100	100
FoxP3	FJ664148	650	100	100	100
TNF-α	AF046215	600	100	100	100
IL-12p40	AF046211	750	100	100	100
γ -actin	FJ664149	-	-	-	-

ISSN: 1471-2172