Expression of FoxP3 mRNA in PBMCs or CD4+ T cells treated with resistin. (A) PBMC and (B) CD4+ T cells isolated from human adult peripheral blood were treated with various concentrations of resistin (0, 50, 100, 200 or 500 ng/ml) for 6 hrs. For measurement of FoxP3 and TGF-β mRNA expression, RT-PCR was performed as described in Materials and Methods. (C) The intensities of the FoxP3 were normalized to β-actin. Results are representative of three separate experiments with similar results.