Figure 3
From: Resistin enhances the expansion of regulatory T cells through modulation of dendritic cells
Resistin suppressed the expression of IRF-1 and cytokines in DCs. The expression of IRF-1, in both (A) mRNA and (B) protein was determined in DCs treated with 500 ng/ml of resistin for the indicated time periods (upper panel in A and B) and treated with indicated amount for 24 hrs (bottom panel in A and B). The heat-inactivated resistin was used as a control. (C) The expression of IL-12p40, IL-23p19, IL-6, TGF-β and IL-10 was measured by RT-PCR in DCs treated with 500 ng/ml of resistin for 12 hrs. The graphs show the intensity of each cytokine normalized to β-actin. Results are representative of three separate experiments with similar result.