Figure 3From: The antigen presentation function of bone marrow-derived mast cells is spatiotemporally restricted to a subset expressing high levels of cell surface FcεRI and MHC IIBlock of surface FcεRI-mediated antigen focusing and presentation of anti-DNP IgE/TNP-OVA by ragweed-specific IgE. BMMC were preincubated with anti-ragweed IgE at 100 μg/ml for one hr, washed and, followed by addition of IgE/TNP-OVA complexes, and coincubated with 3Do-54.8. Levels of IL-2 in 72 hr supernatants were determined by stimulating 3H-thymidine incorporation and proliferation of IL-2 dependent NK-3 cells as described in Material and methods.Back to article page