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Figure 5 | BMC Immunology

Figure 5

From: The antigen presentation function of bone marrow-derived mast cells is spatiotemporally restricted to a subset expressing high levels of cell surface FcεRI and MHC II

Figure 5

Surface FcεRIhi/MHCII(+) BMMC but not surface FcεRIlo/MHCII(-) BMMC are capable of presenting antigens. Three-week old BMMC were sorted into FcεRIhi/MHCII positive vs FcεRIlo/MHCII negative subsets. Each BMMC subset was then incubated with 3Do-54.8 in the presence of IgE-TNP-OVA (Panel A), TNP-OVA (Panel B) and OVA peptide (Panel C), and levels of IL-2 in 72 hr supernatants were determined by stimulating 3H-thymidine incorporation and proliferation of IL-2 dependent NK-3 cells as described in Material and methods.

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BMC Immunology

ISSN: 1471-2172

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