Figure 3
From: Adenovirus F protein as a delivery vehicle for botulinum B
Nasal immunization with Hcβtre-Ad2F plus CT enhances (A) IgA AFC responses in nasal passages (NPs) and NALT and (C) IgG AFC responses in head and neck LNs (HNLNs), submaxillary glands (SMGs), spleens, and Peyer's patches (PPs) when compared with Hcβtre plus CT. Four weeks post-primary immunization, mononuclear cells from selected lymphoid tissues were evaluated by using B cell ELISPOT assay to determine the number and distribution of (A,C) Hcβtre-specific and total (B,D) IgG and IgG AFCs. Data are expressed as mean ± SEM of three separate experiments (n = 15 mice) of AFC/1 × 106 lymphocytes. *, p ≤ 0.001; **, p ≤ 0.05 represents the statistical differences in number of IgA and IgG AFCs in individual tissues between mice immunized with Hcβtre-Ad2F and Hcβtre with CT.