Figure 4

I.m. immunization with Hcβtre-Ad2F plus CT enhances the number of IgG AFCs in peripheral LNs (PLNs) HNLNs, and PPs when compared to mice immunized with Hcβtre plus CT. Four weeks post-primary immunization, mononuclear cells from selected lymphoid tissues were examined using B cell ELISPOT assay to determine the number and distribution of (A, C) Hcβtre-specific and (B, D) total IgA and IgG AFCs. Data are expressed as mean ± SEM of three separate experiments (n = 15 mice) of AFC/1 × 10⁶ lymphocytes. *, p ≤ 0.001; **, p ≤ 0.05 represents the significant differences in number IgG AFCs in individual tissues between mice immunized with Hcβtre-Ad2F and Hcβtre with CT.