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Table 1 Plasma serum amyloid A and cytokine data from Il10-/- and C57 mice

From: Changes in colon gene expression associated with increased colon inflammation in interleukin-10 gene-deficient mice inoculated with Enterococcus species

  

Mouse strain

P(Il10-/-vs. C57)

Assay

Treatment Group

Il10 -/-

C57

 

SAA

SPF

60.1 ± 30.4

7.1 ± 6.6

< 0.05

 

C

43.9 ± 24.4

6.5 ± 4.7

< 0.05

 

EF

140.5 ± 136.3

6.4 ± 7.5

< 0.05

 

CIF

43.5 ± 11.4

6.5 ± 14.2

< 0.05

 

EF·CIF

137.4 ± 161.1

50.6 ± 90.9

< 0.05

IL-1α

SPF

40.4 ± 39.6

25.0 ± 34.6

NS

 

C

11.3 ± 25.2

38.3 ± 36.3

NS

 

EF

a 80.3 ± 67.5

9.5 ± 21.2

0.03

 

CIF

0.0 ± 0.0

55.2 ± 59.4

NS

 

EF·CIF

44.9 ± 49.1

55.4 ± 59.6

NS

IL-4

SPF

1.0 ± 1.0

1.8 ± 1.1

NS

 

C

1.0 ± 0.9

1.0 ± 0.5

NS

 

EF

b 0.3 ± 0.6

b 0.5 ± 0.4

NS

 

CIF

1.0 ± 1.9

1.0 ± 0.5

NS

 

EF·CIF

c 0.4 ± 0.5

c 0.8 ± 1.6

NS

IL-5

SPF

33.4 ± 0.6

59.6 ± 57.0

NS

 

C

19.9 ± 18.2

33.2 ± 1.7

NS

 

EF

31.1 ± 19.3

35.9 ± 3.3

NS

 

CIF

55.4 ± 68.6

26.7 ± 15.0

NS

 

EF·CIF

22.1 ± 20.4

d 13.2 ± 18.1

NS

IL-6

SPF

2.4 ± 3.3

0.0 ± 0

NS

 

C

5.2 ± 11.5

6.1 ± 6.8

NS

 

EF

e 59.7 ± 30.0

5.4 ± 8.4

0.03

 

CIF

8.0 ± 16.0

5.9 ± 8.9

NS

 

EF·CIF

25.3 ± 24.5

0.03 ± 0.08

0.03

  1. Data represent mean ± SD of plasma levels of SAA and IL-6 for 5 mice (except for Il10-/-mice in the CIF group, n = 4) fed AIN-76A diet, and in treatment groups described in the legend to Table 3. P-values are for across-group comparisons between Il10-/-and C57 mice under the same treatment conditions (NS denotes not significantly different between strains). Superscript letters denote mice with values significantly different (P < 0.05) from mice of the same strain in different treatment groups as follows: (a) EF-inoculated Il10-/-mice showed significantly higher IL-1α levels than Il10-/-mice in the C and CIF groups; (b) IL-4 in the EF groups was significantly lower than in the SPF and C groups in both mouse strains; (c) IL-4 in the EF·CIF group was significantly lower than that in the SPF group in both mouse strains; (d) across the C57 mice, those receiving the EF·CIF inoculation had significantly lower IL-5 levels than either EF or SPF; (e) Il10-/-mice in the EF inoculation group had significantly higher IL-6 than all other Il10-/-treatment groups except for EF·CIF.