Figure 2
From: Peripheral blood T Regulatory cell counts may not predict transplant rejection
Pharmacokinetics of RAPA in blood samples obtained from recipients. The extractions of RAPA from blood samples were injected onto tandem supelcosil C18 columns (Supelco, Bellefonte, PA) maintained at 40°C and eluted with mobile phase buffer composed of a 90:10 mixture of methanol:water at a flow rate of 0.5 ml/minute. UV absorbance was monitored at 276 nm. We found that the concentration of RAPA was below its effective concentration (5-15 ng/ml) 5 days after treatment.