Figure 5

Time course and localization of Foxp3+ Tregs in spleens and lymph nodes assessed by immunohistochemistry. We assessed the dynamic changes in the number of Foxp3+ Tregs in the spleens by immunohistochemistry. Tissue sections were stained using an anti-Foxp3 monoclonal antibody. The positive cells in the spleens were counted in 10 ocular grid areas within each section at a magnification of ×200, and total was divided by the number of ocular grid areas. We counted no less than 6 sections in one time point. We assessed the dynamic changes in the numbers of Foxp3+ Tregs in lymph nodes (LN) by immunohistochemistry. Tissue sections were stained using an anti-Foxp3 monoclonal antibody. The positive cells in spleens were counted in 5 ocular grid areas within each section at a magnification of ×200, and total was divided by the number of ocular grid areas. We counted no less than 6 sections in one time point. Con group POD 1: 1^st day after transplantation; POD 3: 3^rd days after transplantation; POD6: 6^th day after transplantation; 1 D group 1+1d: 1^st day after RAPA treatment in 1D group; 1+3d: 3^rd day after RAPA treatment in 1D group; 1+7d: 7^th day after RAPA treatment in 1D group; 1+10d: 10^th day after RAPA treatment in 1D group; 3D group 3+1d: 1^st day after RAPA treatment in 3D group; 3+3d: 3^rd day after RAPA treatment in 3D group; 3+7d: 7^th day after RAPA treatment in 3D group; 3+11d: 11^th day after RAPA treatment in 3D group.