Table 1 Histochemical (L.M.) Antibody Information.
Primary Antibody | Type, Species, IgG-type (Clone) | Dilution | Slide Pretreatment | Source | Secondary antibody | Dilution | Source |
|---|---|---|---|---|---|---|---|
von Willebrand Factor | Polyclonal, Rabbit anti-Human IgG | 1:2000 | P2 = 16 min | Dako, A 0082 | Universal link | Prediluted | Ventana Medical Systems |
Collagen I | Polyclonal, Rabbit anti-Human IgG | 1:100 | (HIER) EDTA 5 min & P2 = 16 min | Biogenesis, 2150-0020 | Universal link | Prediluted | Ventana Medical Systems |
Collagen III | Polyclonal, Rabbit anti-Human IgG | 1:100 | (HIER) EDTA 5 min & P2 = 16 min | Biogenesis, 2150-0100 | Universal link | Prediluted | Ventana Medical Systems |
Laminin | Polyclonal, Rabbit anti-Mouse IgG | 1:25 | P2 = 32 min | Cappel, 10765 | Universal link | Prediluted | Ventana Medical Systems |
Fibronectin | Monoclonal, Mouse anti-Human IgG1 (Clone 568) | 1:100 | P2 = 32 min | Novo Castra, NCL-FIB | Universal link | Prediluted | Ventana Medical Systems |
Collagen IV | Monoclonal, Mouse anti-Human IgG1 (Clone CIV 22(1)) | 1:100 | P2 = 16 min | Dako, M0785 | Universal link | Prediluted | Ventana Medical Systems |