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Figure 3 | BMC Immunology

Figure 3

From: Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases

Figure 3

Biochemical analysis of rhC2. A) Chromatogram representing the purity of rhC2 by size exclusion chromatography: purity was calculated to be ~96%. The % area (% purity) was quantified using Exponential Skim Integration of the Empower software. B) Differential scanning calorimetry: 400 μg/ml of protein were loaded in the calorimetry cell and heated at a rate of 60°C/hour. The excess heat capacity vs. temperature shows a single unfolding transition with a midpoint at (Tm) of 53.4°C.

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