Skip to main content
Figure 4 | BMC Immunology

Figure 4

From: Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases

Figure 4

rhC2 characterization. A) Cleavage of rhC2 by complement protease C1s. Lanes: 1. High molecular weight marker, 2. Low molecular weight marker, 3. rhC2 undigested, 4. rhC2 (25 μg/ml) digested with C1s, 5. rhC2 (50 μg/ml) digested with C1s, 6. plasma purified C2, 7. plasma purified C2 (25 μg/ml) digested with C1s, 8. Low molecular weight marker, 9. High molecular weight marker. B) Reconstitution of complement activity as measured by complement activation ELISA by rhC2 in C2 affinity depleted human serum. Normal human serum was used as standard (100%). Dose response of rhC2 in serum shows activation of the classical pathway at a concentration as low as 3 μg/ml. As negative control, an irrelevant protein (C) was run to confirm specificity of the assay to rhC2. Data represent the mean ± SD of three independent experiments.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us