Figure 4

Effects of LIMK1-WT or LIMK1-DN expression on NADPH oxidase activity. Ra2 cells expressing LIMK1-WT (A,D) or LIMK1-DN (B,E) at increasing protein levels were stimulated with either FMLP (A-C) or PMA (D-F) and superoxide production measured by Luminol E-CL. The graphs represent traces from individual wells of one experiment, where the ordinate shows luminol E-CL normalized to control cells in arbitrary units; arrows indicate time point of agonist injection. The bar graphs shows peak luminol E-CL response for FMLP (C) or PMA (F) stimulated superoxide production, and represents mean and SD of at least three independent experiments. Differences statistically significant at the P < 0.05 (one sample t-test) level are indicated.