Figure 2

Time schedule of re-challenge with 5 × 10⁵viable B16F1 tumor cells of pre-vaccinated mice surviving the first tumor challenge. * vaccination started 90 days before the first injection of viable tumor cells; the vaccine was applied once (1×), twice (2×) or three times (3×) in 15 days interval ** vaccination started 60 days before the first injection of viable tumor cells; the vaccine was applied once (1×), twice (2×) or three times (3×) in 15 days interval *** vaccination started 30 days before the first injection of viable tumor cells; the vaccine was applied once (1×), twice (2×) in 15 days interval or three times (3×) in 15 days interval, except for the last vaccination, which was performed 7 days after second vaccination.