Figure 2
Effect of 10% human serum on the ability of Mip to induce TNF-α and IL-8 production by primary human differentiated macrophages. Human monocytes/macrophages (5 × 105 cells/0.5 ml per well) were differentiated in a 6-day culture in the presence of 2 ng/ml M-CSF and then stimulated by recombinant Mip (1 μg/ml) in the presence or absence of 10% heat-inactivated human serum. After 4 h stimulation, supernatants were collected and their content in TNF-α and IL-8 were analyzed by ELISA. Each value represents the mean ± SD of triplicates from two experiments. The results are expressed as percent of TNF-α or IL-8 release in the absence of serum. ***: p < 0.0001; **: p < 0.005 determined by comparison with medium alone using Student's t test.