Figure 6
Effect of 10% FBS and human serum on TNF-α productions by primary human differentiated macrophages in response to OspA, E. coli LPS, Pam 2 CSK 4 , and Pam 3 CSK 4 and effect of 10 μg/ml apoB on TNF-α and IL-8 production induced by OspA and E. coli LPS. Human monocytes/macrophages (5 × 105 cells/0.5 ml per well) were differentiated in a 6-day culture in the presence of 2 ng/ml M-CSF and then stimulated by recombinant OspA or purified E. coli LPS, both at 1 μg/ml, or 0.01 μg/ml Pam2CSK4 or Pam3CSK4 in the presence of 10% heat-inactivated FBS or human serum (A) or in the presence of 10 μg/ml human plasma apoB (B and C). After 4 h stimulation, supernatants were collected and their content in TNF-α and IL-8 were analyzed by ELISA. The results are expressed as percent of TNF-α or IL-8 release in presence of 10% FBS. Each value represents the mean ± SD of triplicates from two experiments. **: p < 0.005, ***: p < 0.0001 determined by comparison with 10% FBS using Student's t test.