Figure 1From: Bystander T cells in human immune responses to dengue antigensIFN-γ induction by inactivated dengue virus serotype 2 of 55 healthy Thai children aged 13-14 years. Whole blood samples containing 9 × 105 lymphocytes/ml were co-cultured with medium alone, Den2, PHA and IL-12 plus IL-15 in the presence or absence of 0.3 μg/ml cyclosporin A (CsA) for 48 h. The cultured supernatants were assayed for IFN-γ by sandwich ELISA (A). Percentages of IFN-γ inhibition as calculated by CsA treatment (B). *** represents the statistically significant difference between the two groups (P < 0.0001, Mann Whitney test; NS represents non significant).Back to article page