Figure 3

Kinetics of TCR signaling-specific vs. bystander IFN-γ producing CD4⁺T and CD8⁺T cell subsets. The cultured cells from seven representative samples were cultured with Den2 and analyzed the frequencies of IFN-γ⁺ CD4⁺ or CD8⁺ T cells in the presence of CsA (bystander T cells) or NK cells at 12, 24 and 36 h (A). The kinetics of specific vs. bystander CD4⁺ or CD8⁺ T cells (B). The distributions of specific vs. bystander CD4⁺ or CD8⁺ cells activated with Den2 for 24 h from 11 samples were compared using the Mann Whitney test (NS represents non significant). The isotype controls were also stained and showed the background of % IFN-γ producing cells less than 0.01%.