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Figure 2 | BMC Immunology

Figure 2

From: Macrophage migration inhibitory factor regulates interleukin-6 production by facilitating nuclear factor-kappa B activation during Vibrio vulnificus infection

Figure 2

The effects of ISO-1 on IL-6, IL-8, and TNF-α production in V. vulnificus -infected whole blood and isolated PBMCs. Cells pre-treated with different doses of ISO-1 were infected with V. vulnificus (Vv05191; MOI = 1) for the indicated time periods. After plasma and supernatants had been collected, ELISA was used to determine IL-6 (A), IL-8 (B), TNF-α (C), and MIF (D) production. (E) With or without anti-MIF neutralising antibodies, ELISA was used to measure the concentrations of TNF-α, IL-6, and IL-8 in V. vulnificus-infected PBMCs at 4 h post-infection. Control goat IgG and ISO-1 were used as negative and positive controls, respectively. Data are the means ± SD from three individual experiments. *P < 0.05 vs. V. vulnificus infection only.

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BMC Immunology

ISSN: 1471-2172

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