qRT-PCR validation of microarray data. (A) CXCR3, chemokine (C-X-C) receptor 3; (B) GNLY, granulysin; (C) TRAF3, TNF receptor-associated factor 3; (D) TLR6, toll-like receptor 6; (E) SLA, src-like-adaptor; (F) Novel Gene, no RefSeq; (G) LOC509457, predicted WD repeat domain 73. Gene expression assessed by qRT-PCR (black triangle) is expressed relative to the PI level. A time course of microarray data (black square) was calculated from comparative values and is expressed relative to the PI level. Comparative values used for this calculation are tabulated. aDifferential expression of genes in pooled samples PI_I1, PI_I2, PI_I3, I1_I2, I1_I3, I2_I3 or PI_C1, PI_C2, PI_C3, C1_C2, C1_C3, C2_C3 where treated as described in Methods. Down regulated genes with more than a 1.3-fold decrease (-1.3) and a P value < 0.05 are highlighted green. Up-regulated genes with > 1.3-fold increase and a P value < 0.05 are highlighted red. Genes where the change is less than a 1.3-fold decrease or increase and with a P value < 0.05 are highlighted grey. The procedure and reagents used are described in Methods.