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Table 2 Oligonucleotide sequences of ovine GAPDH and a selected range of genes employed for SYBR Green real time PCR

From: The gastrointestinal nematode Trichostrongylus colubriformis down-regulates immune gene expression in migratory cells in afferent lymph

Gene

Forward primer (5'-3')

Reverse primer (5'-3')

GAPDH

CACCATCTTCCAGGAGCGAG

CCAGCATCACCCCACTTGAT

Novel Gene

CAAGCTAAAGGCAGCATCCC

TCTCCCTCATAAGCCTGGAGC

GNLY

GGTCTGCAAAAGCAAGGCAG

TCAGAGGACCCAGGGAATCA

SLA

ACCACGGTTGGCTGTTTGAA

GCAGCTCCTCAGCCTTGTCT

CXCR3

GTGCTGACACTCCCTCTCTGG

AAAGACCCACTGGATGGCTG

TRAF3

CTTCTGTGAGACCTGCATGGG

CATTTTGGGCTGGAGGAGC

TLR6

AATGACTTTGATGCCCTGCC

CTGGGTCAAGTTGCCAAATTC

LOC509457

AAGGGATACGGGAACTTGGC

AAAGGGCTTCATTGCTGAGC

  1. aPrimers were designed to the ovine homologues of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), the src-like adaptor (SLA), chemokine (C-X-C) receptor 3 (CXCR3), TNF receptor-associated factor 3 (TRAF3), toll-like receptor 6 (TLR6), granulysin (GNLY), predicted WD repeat domain 73 (LOC509457) and a novel ovine EST (Novel Gene).
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BMC Immunology

ISSN: 1471-2172

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