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Figure 3 | BMC Immunology

Figure 3

From: Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophages

Figure 3

ST2 protein expression in human bone marrow-derived macrophages. (A-H) Immunostaining of human macrophages cultured on Lab-Tek slides after exposure to medium alone (A & E), IL-33 (B & F), M1 polarizing mediators (C & G), or M2 polarizing mediators (D & H). IgG controls are shown in panels A, B, C, & D. (I) Western blotting for ST2L and sST2 protein was performed in protein extracts from naïve and M2-polarized human macrophages; CE = cytoplasmic extract and NE = nuclear extract. (J) Quantitative analysis of ST2L and sST2 protein band intensity.

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