Figure 3From: Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophagesST2 protein expression in human bone marrow-derived macrophages. (A-H) Immunostaining of human macrophages cultured on Lab-Tek slides after exposure to medium alone (A & E), IL-33 (B & F), M1 polarizing mediators (C & G), or M2 polarizing mediators (D & H). IgG controls are shown in panels A, B, C, & D. (I) Western blotting for ST2L and sST2 protein was performed in protein extracts from naïve and M2-polarized human macrophages; CE = cytoplasmic extract and NE = nuclear extract. (J) Quantitative analysis of ST2L and sST2 protein band intensity.Back to article page