Figure 5

Effect of IL-33 on human macrophage polarization. (A) CCL18 and CCL3 transcript expression in macrophages following exposure to IL-33 (10 ng/ml) alone for 24 h. (B) CCL18 and CCL3 transcript expression in human macrophages following exposure to M1 polarizing mediators + IL-33 (10 ng/ml) for 24 h. (C) CCL18 and CCL3 transcript expression in human macrophages following exposure to M2 polarizing mediators + IL-33 (10 ng/ml) for 24 h. Results are expressed as fold increase in transcript levels compared with levels of these transcripts in human macrophages exposed to media alone (i.e. control condition). Data are shown as mean ± SEM and are representative of three separate experiments with 3 donor macrophage lines. * P ≤ 0.05 compared with transcript levels in appropriate control macrophages.