Figure 3

Expression of PAR1- and PAR2-induced innate immunity is enhanced when PI3K/Akt signaling pathway is blocked. HOKs were incubated with inhibitors for PI3K (Wortmannin) at 25-400 nM (a-b) or for Akt (Akt inhibitor IV) at 0.5-2.5 uM (c-d) for 1 h and subsequently stimulated with thrombin (10 U/ml) or trypsin (10 nM) for 6 h. Cells were harvested and expression of the selected markers was assessed by QRT-PCR. Data from three separate donors set up in duplicates were normalized to GAPDH. Data are given as means of normalized samples to unstimulated control ± SEM. (*p < 0.05 compared to the same condition without inhibitors).