Figure 8
B/I activation is required for proliferation of adoptively transferred tDLN. Mice with established 10 day 4T1 flank tumors received 50 × 106 CFSE-stained lymphocytes, which were either activated with B/I and expanded in culture or untreated ("No B/I"). The mice shown in the upper panel received AIT with cells only; mice in the lower 2 panels received CYP (100 mg/kg i.p.) on day 9 after tumor inoculation. At days 3, 5, 7 and 10 after AIT, the tDLN were harvested from the recipient mice and analyzed by flow cytometry for CFSE+ cells. This is a representative experiment of 3 replicates.