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Table 1 Phenotype of CD62L--enriched T cells from tDLN, before and after B/I pulse and expansion in IL-2

From: Phenotype, functions and fate of adoptively transferred tumor draining lymphocytes activated ex vivo in mice with an aggressive weakly immunogenic mammary carcinoma

% of CD8 cells which express:

Before B/I

After B/I

CD62L +

19%

46%

CD127 +

9%

48%

CD69 +

45%

90%

CD27 +

37%

89%

CCR7 +

46%

42%

  1. 4T1 tDLN were harvested and separated by expression of CD62L. Subsets were activated by B/I and expanded for 7 days. Prior to B/I activation, and again after expansion, cells were stained for expression of CD8, CD62L, CD127, CD69, CD27, and CCR7. The CD62L- fraction was initially 93% CD62L- overall. Shown in the table is the percentage of CD8+ cells which express specific T cell markers, before and after B/I expansion, in the initially CD62L- fraction of tDLN cells. Results are representative of more than three independent experiments.
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BMC Immunology

ISSN: 1471-2172

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