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Table 2 In viv o trafficking and accumulation over time of adoptively transferred cells in tumor-bearing mice

From: Phenotype, functions and fate of adoptively transferred tumor draining lymphocytes activated ex vivo in mice with an aggressive weakly immunogenic mammary carcinoma

 

tDLN

cLN

Tumor

Spleen

Day 0

0.7%

0.7%

0.1%

3.0%

Day 3

9.2%

5.9%

0.2%

10.5%

Day 5

10.1%

7.3%

2.1%

3.1%

Day 7

10.3%

6.5%

0.8%

3.4%

Day 12

8.4%

8.7%

0.1%

2.1%

  1. Mice with established 4T1 flank tumors were either untreated, treated with CYP alone on day 9, or were treated with CYP on Day 9 followed by AIT with 50 × 106 CFSE-stained B/I activated 4T1 tDLN (on day 10). At 1 hour, 3, 5, 7, and 12 days after AIT, the tumors, spleens, tDLN, and contralateral LN (cLN) were harvested and analyzed by flow cytometry for CFSE+ cells. This is a representative experiment of 5 replicates. Percentages of CFSE+ cells found in different tissues at various times after AIT ("0" is 1 hour after infusion of cells) are shown