| tDLN | cLN | Tumor | Spleen |
---|
Day 0
| 0.7% | 0.7% | 0.1% | 3.0% |
Day 3
| 9.2% | 5.9% | 0.2% | 10.5% |
Day 5
| 10.1% | 7.3% | 2.1% | 3.1% |
Day 7
| 10.3% | 6.5% | 0.8% | 3.4% |
Day 12
| 8.4% | 8.7% | 0.1% | 2.1% |
- Mice with established 4T1 flank tumors were either untreated, treated with CYP alone on day 9, or were treated with CYP on Day 9 followed by AIT with 50 × 106 CFSE-stained B/I activated 4T1 tDLN (on day 10). At 1 hour, 3, 5, 7, and 12 days after AIT, the tumors, spleens, tDLN, and contralateral LN (cLN) were harvested and analyzed by flow cytometry for CFSE+ cells. This is a representative experiment of 5 replicates. Percentages of CFSE+ cells found in different tissues at various times after AIT ("0" is 1 hour after infusion of cells) are shown