Figure 1

IgG1 elicited during pre-patent schistosome infection is primarily specific for antigens of 31 kDa. Worm antigen-specific IgM (A), IgG1 (B) and IgG2b (C) in the plasma of wild type mice with pre-patent (2, 3 and 4 weeks post infection) and patent infection (8 weeks post infection) were quantified by ELISA. Data are displayed as relative fluorescence units (RFU). P values for the first time points to show significant increases in antibody level are shown (obtained by Dunn's post-test following Kruskal-Wallis test). Experimental groups consisted of five mice per group. Data are representative of three independent experiments. Triplicate samples of two different SWAP preparations were separated by SDS-PAGE and probed by immunoblotting with plasma from infected mice at 2 (D), 3 (E), 4 (F) and 5 (G) weeks post infection. Bound IgG1 was detected with an alkaline phosphatase-conjugated goat anti-mouse IgG1 antibody. H, SDS-PAGE of three SWAP samples, stained for total protein by Coomassie Blue staining. Positions of protein molecular weight markers are indicated in kDa. Data are representative of three independent experiments.