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Figure 4 | BMC Immunology

Figure 4

From: TGF-β-induced growth inhibition in B-cell lymphoma correlates with Smad1/5 signalling and constitutively active p38 MAPK

Figure 4

Pai-1 and Id1 are up-regulated upon TGF-β treatment. (A) Cell lines were stimulated with or without TGF-β for 1, 6 or 24 h, before total RNA was isolated, cDNA was synthesized and real-time RT-PCR was conducted with Pai-1 as target gene. Illustrated is relative Pai-1 mRNA expression (one representative experiment of three, ± SD). (B) Cells were stimulated without or with TGF-β for 3 h, lysed, and subjected to western immunoblotting analysis, with anti-Id1 Ab, and anti-actin Ab as loading control. Shown is one representative blot out of three.

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