Figure 3

The flow cytometric histograms of JAWS II DCs and primary murine DCs stained with fluorochrome-conjugated antibodies specific to DC-markers. The primary DCs were harvested on days 2 (2dDC) and 4 (4dDC) of culture and stained with fluorochrome-conjugated antibodies specific to MHC class II, CD11c, CD40, CD80, CD86 antigens. The cell debris was gated-out and histogram charts were plotted. The filled histogram charts are of cells stained with isotype-control antibody. The mean fluorescent intensity (MFI) values were determined for the cells under the bar (⊓) region. The values within the charts indicate the MFI values for the cells stained with isotype-control antibody (I), or antigens-specific antibodies (M). The results are from one representative experiment conducted thrice.