Table 2 List of fluorochrome-conjugated antibodies.
Cells | Fluorochrome-conjugated antibodies for staining DC or lymphocytes |
|---|---|
DCs | Fluorescein isothiocyanate-conjugated (FITC)-CD14, CD3, CD86, CD62L; phycoerythrin (PE)-CD11c, CD45R, CD80, PDCA-1; Biotin or allophycocyanin (APC)-CD40, MHC class II (I-A/I-E), 120G8 and B220. |
Cells from DC-lymphocyte co-culture | FITC-CD69, PE- CD8b (Ly-3), APC-CD4, PerCP-Cy5.5-CD25. |
Splenic lymphocytes, thymocytes and lymph node cells | FITC- CD69, PE-CD127, Pacific orange-CD4, APC-CD8, PerCpCy5.5-CD25, Pacific blue-CD44, APC-Cy7-CD62L, Biotin-CCR7 |