Table 2 Sequences and exon locations of qPCR primers, amplicon lengths, PCR efficiencies and correlation coefficients
Gene Symbol | Reference sequence | Forward primer | location | Reverse primer | location | Amplicon length (bp) | PCR efficiency (%) | R2 |
|---|---|---|---|---|---|---|---|---|
GAPDH | NM_002046 | TGCACCACCAACTGCTTA C | Ex7 | GGCATGGACTGTGGTCATGAG | Ex7-8 | 87 | 109 | 0,99 |
SDHA | NM_004168 | TGGGAACAAGAGGGCATCTG | Ex2 | CCACCACTGCATCAAATTCATG | Ex3 | 86 | 104 | 0,9914 |
HPRT | NM_000194 | TGACACTGGCAAAACAATGCA | Ex5-6 | GGTCCTTTTCACCAGCAAGCT | Ex6 | 94 | 110 | 0,9985 |
IL8 | NM_000584 | GAATGGGTTTGCTAGAATGTGATA | Ex4 | CAGACTAGGGTTGCCAGATTTAAC | Ex4 | 129 | 99 | 0,9995 |
TNF | NM_000594 | CCTGCCCCAATCCCTTTATT | Ex4 | CCCTAAGCCCCCAATTCTCT | Ex4 | 80 | 88 | 0,9991 |
IL17A | NM_002190 | CCATAGTGAAGGCAGGAATC | Ex3 | CGGTTATGGATGTTCAGGTT | Ex3 | 108 | 98 | 0,998 |
IL17F | NM_052872 | AGCGCAACATGACAGTGAAG | Ex1 | GTGTAATTCCAGGGGGAGGT | Ex2 | 105 | 89 | 0,993 |
IL21 | NM_021803 | ACTTGGTCCCTGAATTTCTGC | Ex1-2 | TTTGTGGAAGGTGGTTTCCTC | Ex3 | 169 | 92 | 0,9891 |
IL22 | NM_020525 | TTCCAGCAGCCCTATATCACC | Ex1 | GCTCACTCATACTGACTCCGTG | Ex2-3 | 125 | 102 | 0,9937 |
IL26 | NM_018402 | ATCAAAGCAGCATGGCTCAAA | Ex1 | GCAGTTGACCAAAAACGTCTTCC | Ex3 | 154 | 104 | 0,9894 |
IL1B | NM_000576 | CACGATGCACCTGTACGATCA | Ex5 | GTTGCTCCATATCCTGTCCCT | Ex5 | 121 | 90 | 0,9991 |
IL6 | NM_000600 | ATTCTGCGCAGCTTTAAGGA | Ex5 | AACAACAATCTGAGGTGCCC | Ex5 | 119 | 104 | 0,9956 |
TGFB1 | NM_0006600 | GGCCAGATCCTGTCCAAGC | Ex1 | GTGGGTTTCCACCATTAGCAC | Ex1 | 201 | 93 | 0,9872 |
IL23A | NM_016584 | TTCTGCTTGCAAAGGATCCA | Ex3 | AATATCCGATCCTAGCAGCTTCTC | Ex3 | 64 | 106 | 0,9957 |
STAT3 | NM_139276 | GATCCAGTCCGTGGAACCAT | Ex21 | ATAGCCCATGATGATTTCAGCAA | Ex21 | 74 | 106 | 1 |
CCR6 | NM_031409 | GTGCAAGTTGCTAAAAGGCATC | Ex3 | CGAATGACTTAGTCGCCTGT | Ex3 | 112 | 89 | 1 |
CCL20 | NM_004591 | TGCTGTACCAAGAGTTTGCTC | Ex1 | CGCACACAGACAACTTTTTCTTT | Ex3 | 220 | 98 | 0,9914 |
RORC | NM_005060 | GTAACGCGGCCTACTCCTG | Ex4 | GTCTTGACCACTGGTTCCTGT | Ex5 | 227 | 102 | 0,9944 |
RORC (RORC1) | NM_005060 | GCAAAGAAGACCCACACCTC | Ex2-3 | GCACCCCTCACAGGTGATAA | Ex3 | 102 | 112 | 0,9843 |