Peyer's patch cytokine secretion. Isolated Peyer's patch cells were cultured in vitro with or without anti-CD3/CD28 stimulation. Secretion of TNF-α and IFN-γ was significantly reduced in animals pre-fed B. animalis AHC7 (a). Secretion of IL-10 was unaltered between the groups while a non-statistically significant increase in IL-12 secretion was observed (b). *p < 0.05 versus placebo (n = 10 animals per group).