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Figure 4 | BMC Immunology

Figure 4

From: Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses

Figure 4

Effector CD4+ and CD8+ T cell responses in BALB/c mice immunized with PEI/pci-S complexes. Multi-intracellular cytokine staining for IL-17, IFN-γ, TNF-α and IL-2 was performed on (A) CD4+ and (B) CD8+ T cells after in vitro re-stimulation with SARS peptide. SARS S-specific CD4+ T cells from lung were recovered on day 6 after the last immunization. ND indicates not detectable. Data were expressed as the mean value of mean fluorescence intensity ± SEM. Significant differences compared with pci-S group were expressed as *P < 0.01. (C) The results showed representative example of flow cytometry analysis.

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