Figure 1
From: Subolesin expression in response to pathogen infection in ticks
Correlation between subolesin expression and A. marginale infection levels in D. variabilis male guts and salivary glands. RNA was extracted from guts collected after acquisition feeding (D-E) and salivary glands collected after transmission feeding (A-C) in 5 pools of 10 ticks each of D. variabilis (A and D), D. andersoni (B and E) and R. sanguineus (C and F) male ticks experimentally infected with A. marginale. Subolesin and msp4 mRNA levels were analyzed by real-time RT-PCR and normalized against tick 16S rRNA using the comparative Ct method [9, 32]. Regression analyses were conducted in Microsoft Excel to compare normalized A. marginale msp4 and subolesin mRNA levels.