Table 1 Immunisation protocol and time points for collection of blood and spleens.
Host | Time-points | Maternal treatment | |||
|---|---|---|---|---|---|
PT mothers | CpG mothers | Al(OH)3 mothers | Naive mothers | ||
Mothers | Day 3 of pregnancy. | Blood samples (T0) | |||
10 μg OVA + 0.5 μg PT N = 10 | 10 μg OVA + 50 μg CpG N = 10 | 10 μg OVA + 2 mg Al(OH)3 N = 10 | Not immunised N = 10 | ||
Day 10 of pregnancy | 1 μg OVA | 1 μg OVA + 5 μg CpG | 1 μg OVA + 0.2 mg Al(OH)3 | Not immunised | |
Day 10 after delivery | Blood samples (T10) | ||||
3 weeks after delivery | Exsanguinated (TT): serum and spleens collected | ||||
Offspring | 6 weeks | Blood samples (T0) Immunised: 10 μg OVA + 2 mg Al(OH)3 N = 40 offspring per maternal treatment group | |||
8 weeks | 10 μg OVA | ||||
9 weeks | Exsanguinated (TT): serum and spleen cells were collected | ||||