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Figure 5 | BMC Immunology

Figure 5

From: Mannan-binding lectin regulates dendritic cell maturation and cytokine production induced by lipopolysaccharide

Figure 5

MBL attenuates the binding of smooth LPS to imMDCs. ImMDCs were preincubated with (black solid line) or without (dotted line) MBL (10 μg/ml) for 30 min (A), or with anti-MBL pAb (30 μg/ml) and MBL (black solid line) for 30 min (B), and further incubated at 4°C for 30 min with Alexa488-labeled smooth LPS (E. coli O111: B4). The binding of LPS on the cell surface was determined by FACScan. The histograms shown are representatives from three experiments. Shaded curves, the negative control without labeled LPS. (C) Mean fluorescence intensity, data given as mean ± S.D. from three independent experiments. *P < 0.05 as compared to LPS group.

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