Figure 6

Ca²⁺-dependent binding of MBL to imMDCs. (A) Tris-buffered saline with 1.5 mM CaCl₂; (B) Tris-buffered saline with 5 mM CaCl₂; (C) Tris-buffered saline with 10 mM CaCl₂; (D) Tris-buffered saline with 5 mM EDTA. Each solution of imMDCs was added with the biotinylated MBL (10 μg/ml) for 10 min incubation before addition of ExtrAvidin-FITC. After incubation for 30 min on ice, binding of MBL to the cells was analyzed by FACScan, as shown in the representative histograms (A-D). Black lines, biotinylated MBL binding; dotted lines, negative controls (the cells only). These data are representative of four independent experiments.