Figure 1

BJcuL is evenly distributed on the surface of human neutrophils. Isolated human neutrophils (10⁶ cells/ml) were adhered to coverslips coated with Biobond, fixed and then incubated for 45 min at RT with (A) PBS; (B) Biotinylated BJcuL (10 μg/ml); (C) Biotinylated BJcuL pre-incubated with Gal 2 mM. (D) Biotinylated BJcuL pre-incubated with GlcNAc 2 mM. Cells were examined by fluorescence microscopy after reaction with streptavidin-FITC.