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Figure 3 | BMC Immunology

Figure 3

From: Human neutrophil migration and activation by BJcuL, a galactose binding lectin purified from Bothrops jararacussu venom

Figure 3

BJcuL was able to induce neutrophil migration. Neutrophil chemotaxis assays were carried out in 48-well Boyden microchambers. BJcuL (2.5 ng/ml), pretreated or not Gal or GlcNAc was placed in the lower chamber wells; RPMI was used as negative control. The wells of the upper chamber received isolated neutrophils (1 × 106 cells/well). The number of cells that migrated through the filter separating the two chambers was scored in each field. The figure is representative of the results of three different experiments and expressed as mean ± SD (p < 0.05, Student's t-test).

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