Figure 5

BJcuL stimulation enhances neutrophil functions. Following 30 min at 37°C of incubation with BJcuL (2.5 μg/ml), PMA (1 μg/ml), or CXCL-8 (2 μg/ml), neutrophil functions were evaluated. Control cells were incubated with PBS only. (A) Neutral red-stained zymozan was used as a target for the phagocytic activity of stimulated neutrophils. (B) Neutral red uptake was used to assess the volume of the neutrophil lysosomal system. (C) Superoxide production was determined by the reduction of NBT. The results are shown as mean ± SD and expressed as absorbance reading at the indicated wavelength. The values are representative of three different experiments. All stimuli provided responses that were statistically different (p < 0.05, Student's t-test) from the control.